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| Registros recuperados: 43 | |
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| Barbosa,Aulus Estevão; Marinho,Paulo. |
| The Eucalyptus Genome Sequencing Project (FORESTs), an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis), has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX). Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y) and cytosolic (TRXh), have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Transcriptome; Thioredoxin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400008 |
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| Foulon, Valentin; Boudry, Pierre; Artigaud, Sebastien; Guérard, Fabienne; Hellio, Claire. |
| Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Pediveliger larvae; Bioadhesive; Transcriptome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00475/58677/61172.pdf |
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| Sousa,Cristina Soares de; Serrão,José Eduardo; Bonetti,Ana Maria; Amaral,Isabel Marques Rodrigues; Kerr,Warwick Estevam; Maranhão,Andréa Queiroz; Ueira-Vieira,Carlos. |
| The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fat body; Gene expression; Melipona; Stingless bees; Transcriptome. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022 |
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| Rodrigues, Sophie; Paillard, Christine; Van Dillen, Sabine; Tahrioui, Ali; Berjeaud, Jean-marc; Dufour, Alain; Bazire, Alexis. |
| Marine pathogenic bacteria are able to form biofilms on many surfaces, such as mollusc shells, and they can wait for the appropriate opportunity to induce their virulence. Vibrio tapetis can develop such biofilms on the inner surface of shells of the Ruditapes philippinarum clam, leading to the formation of a brown conchiolin deposit in the form of a ring, hence the name of the disease: Brown Ring Disease. The virulence of V. tapetis is presumed to be related to its capacity to form biofilms, but the link has never been clearly established at the physiological or genetic level. In the present study, we used RNA-seq analysis to identify biofilm- and virulence-related genes displaying altered expression in biofilms compared to the planktonic condition. A... |
| Tipo: Text |
Palavras-chave: Biofilm; Virulence; Vibrio tapetis; Transcriptome; Quorum sensing; Type VI secretion system; Brown ring disease. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00470/58140/60611.pdf |
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| Xiaojun,Fan; Chun,Yang; Jianhong,Liu; Chang,Zhang; Yao,Li. |
| Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Asian gypsy moth; Chitin metabolism; Transcriptome; Unigenes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100160 |
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| Rodrigues,Fabiana Aparecida; Marcolino-Gomes,Juliana; Carvalho,Josirlei de Fátima Corrêa; Nascimento,Leandro Costa do; Neumaier,Norman; Farias,José Renato Bouças; Carazzolle,Marcelo Falsarella; Marcelino,Francismar Corrêa; Nepomuceno,Alexandre Lima. |
| Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Drought; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
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| Pratlong, M.; Haguenauer, A.; Chabrol, O.; Klopp, C.; Pontarotti, P.; Aurelle, D.. |
| The question of species survival and evolution in heterogeneous environments has long been a subject for study. Indeed, it is often difficult to identify the molecular basis of adaptation to contrasted environments, and nongenetic effects increase the difficulty to disentangle fixed effects, such as genetic adaptation, from variable effects, such as individual phenotypic plasticity, in adaptation. Nevertheless, this question is also of great importance for understanding the evolution of species in a context of climate change. The red coral (Corallium rubrum) lives in the Mediterranean Sea, where at depths ranging from 5 to 600m, it meets very contrasted thermal conditions. The shallowest populations of this species suffered from mortality events linked... |
| Tipo: Text |
Palavras-chave: Cnidarians; Gene expression; Local adaptation; Octocoral polymorphism; Transcriptome. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00371/48257/48661.pdf |
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| SALGADO, F. F.; GRYNBERG, P.; SOUZA JUNIOR, M. T.. |
| The discovery of noncoding RNAs (ncRNAs) is changed our understanding of genome, transcriptome, and interactions with proteome in a cell. Since the discovery of the first microRNA (miRNA) called lin-4 on Caenorhabditis elegans in 1993, our understanding of gene expression has deepened. Further miRNA discoveries have led to the investigation of new ncRNA families and their function, biogenesis, and interactions with other types of molecules. Recent developments in bioinformatics, public databases, sequencing technologies, and biochemical techniques have increased our understanding of the biogenesis, function, and interactions of ncRNAs. This book provides an overview of current ncRNA research dealing with human diseases and cancer and plant miRNAs. |
| Tipo: Parte de livro |
Palavras-chave: Óleo Vegetal; Oil palm products; Abiotic stress; Salt stress; Transcriptome; Oilseed crops. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148820 |
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| Ma,Juan; Gao,Bo; Wang,Rongyan; Li,Xiuhua; Chen,Shulong. |
| Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potato rot nematode; Transcriptome; Tolerance; Differentially expressed genes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100202 |
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| He,Ruishi; Zhu,Xinxin; Li,Qiaoyun; Jiang,Yumei; Yu,Dongyan; Sun,Yulong; Liang,Xiaolong; Ni,Yongjing; Niu,Jishan. |
| ABSTRACT: Wheat (Triticum aestivum L.) stem development significantly affects grain yield. The dwarf plants (D) of wheat mutant dms was less than 30cm. Here, we were to explore the molecular basis for the restrained stem development of the dwarf plants. The results were reached by compare the young spikes and stems transcriptomes of the tall (T) and D plants of mutant dms. We identified 663 genes highly expressed in stem tips. We identified 997 differentially expressed genes (DEGs) in stem tips between T and D, 403 DEGs were significantly related with stem development. Most biological processes in stem tips on dwarf plants were significantly suppressed, such as phytohormone signaling etc. The sequencing analysis results were confirmed by quantitatively... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wheat (Triticum aestivum L.); Dms mutant; Transcriptome; Stem development.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200401 |
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| Sá,Maria Eugênia Lisei de; Lopes,Marcus José Conceição; Campos,Magnólia de Araújo; Paiva,Luciano Vilela; Amorim,Regina Maria Santos de; Beneventi,Magda Aparecida; Firmino,Alexandre Augusto Pereira; Sá,Maria Fátima Grossi de. |
| Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Root knot nematode; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200008 |
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| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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| Ellison, Amy R.; Uren Webster, Tamsyn M.; Rey, Olivier; Garcia De Leaniz, Carlos; Consuegra, Sofia; Orozco-terwengel, Pablo; Cable, Jo. |
| Background: Captive animal populations, be it for food production or conservation programmes, are often maintained at densities far beyond those in natural environments, which can have profound effects on behaviour, immune and stress levels, and ultimately welfare. How such alterations impact transcriptional responses to pathogen infection is a 'different kettle of fish' and remains poorly understood. Here, we assessed survival and gene expression profiles of infected fish reared at two different densities to elucidate potential functional genomic mechanisms for density-related differences in disease susceptibility. Results: Utilising a whole-transcriptome sequencing (RNAseq) approach, we demonstrate that rearing density in tilapia (Oreochromis niloticus)... |
| Tipo: Text |
Palavras-chave: Stocking density; Stress; Saprolegnia; Aquaculture; Transcriptome; Tilapia; Circadian rhythm; Disease risk. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00462/57353/78952.pdf |
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| Registros recuperados: 43 | |
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